High broodstock fads2 expression combined with nutritional programing through broodstock diet improves the use of low fishmeal and low fish oil diets in gilthead seabream (Sparus aurata) progeny

Abstract One of the factors that limits replacement fish meal (FM) and oil (FO) by plant ingredients in diets for marine fish, is their lack long chain-polyunsaturated fatty acids (LC-PUFA). LC-PUFA are essential these species, which sufficient acyl desaturase 2 (Fads2) activity to synthesize them. Nutritional programing or use broodstock with a higher Fads2 could improve ability low FM FO diets. The aim this study was determine effect gilthead seabream inherently high fads2 gene expression nutritional rich rapeseed (RO) on progeny growth performance, liver morphology, biochemical composition selected genes. Sea bream juveniles (2.31± 0.01 g initial body weight, mean ± SD) obtained from either (H) (L) fed diet based RO were randomly distributed into 12 × 250 L tanks nutritionally challenged 45 days containing only 7.5% no FO. highest found diet, whereas lowest those diet. Juveniles showed significantly increased PUFA contents muscle. Replacement led hepatic 18:3n-6/18:2n-6 ratio reduction viscerosomatic index juveniles, hepatocyte size ghr-1/ghr-2 Overall, results significant trans-generational effects both type lipid metabolism performance juvenile